m akt inhibitor viii Search Results


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pan caspase inhibitor z vad fmk  (R&D Systems)
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pan-caspase inhibitor z-vad  (Millipore)
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akt inhibitor viii  (Millipore)
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akt inhibitor viii  (Merck KGaA)
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akt inhibitor 1/2  (Millipore)
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akt inhibitor  (Millipore)
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akt inhibitor  (Selleck Chemicals)
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Selleck Chemicals akt inhibitor
Fig. 2. SLC7A5-dependent late endosomal mTORC1 activation in naïve CD4+ T cell responses. (A and B) Naïve CD4+ T cells from young and older healthy individuals were activated with anti-CD3/anti-CD28 beads for 3 days in the presence of vehicle or <t>SLC7A5</t> <t>inhibitor</t> JPH203 (top). Alternatively, cells were activated with anti-CD3/ anti-CD28 beads for 5 days with the last 2 days in the presence of vehicle or JPH203 (bottom). mTORC1 activities were determined either by Western blotting of p-S6K1 (A) or by flow cytometry of intracellular p-S6RP (S235/S236) (B). Data are shown as one representative experiment (left) and cumulative data of three or four experiments (right). (C) Naïve CD4+ T cells were activated for 3 days followed by treatment or not with an <t>AKT</t> inhibitor for 2 hours before harvesting. Endosomes were isolated and analyzed for in vitro mTORC1 kinase activity toward S6K1. Total cell lysates (T) and endosome isolates (E) were analyzed by immunoblotting for indicated proteins. Data are shown as one representative of three experiments. (D) In vitro mTORC1 kinase activity of endosome isolates in day 5–stimulated naïve CD4+ T cells from one young and one older individual. Data are shown as one representative of three experiments. (E) Cells were stained with anti-EEA1, anti-LAMP1, and anti-mTOR. Confocal images representa- tive of two independent experiments are shown. Scale bars, 5 m. (F and G) mTORC1 activities in day 3– and day 5–stimulated naïve CD4+ T cells from young and older in- dividuals after control or VPS39 silencing. The gray histogram represents isotype control. Comparison by two-tailed paired t test (A, B, F, and G). *P < 0.05 and **P < 0.01.
Akt Inhibitor, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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akt inhibitor ipatasertib  (MedChemExpress)
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MedChemExpress akt inhibitor ipatasertib
Fig. 2. SLC7A5-dependent late endosomal mTORC1 activation in naïve CD4+ T cell responses. (A and B) Naïve CD4+ T cells from young and older healthy individuals were activated with anti-CD3/anti-CD28 beads for 3 days in the presence of vehicle or <t>SLC7A5</t> <t>inhibitor</t> JPH203 (top). Alternatively, cells were activated with anti-CD3/ anti-CD28 beads for 5 days with the last 2 days in the presence of vehicle or JPH203 (bottom). mTORC1 activities were determined either by Western blotting of p-S6K1 (A) or by flow cytometry of intracellular p-S6RP (S235/S236) (B). Data are shown as one representative experiment (left) and cumulative data of three or four experiments (right). (C) Naïve CD4+ T cells were activated for 3 days followed by treatment or not with an <t>AKT</t> inhibitor for 2 hours before harvesting. Endosomes were isolated and analyzed for in vitro mTORC1 kinase activity toward S6K1. Total cell lysates (T) and endosome isolates (E) were analyzed by immunoblotting for indicated proteins. Data are shown as one representative of three experiments. (D) In vitro mTORC1 kinase activity of endosome isolates in day 5–stimulated naïve CD4+ T cells from one young and one older individual. Data are shown as one representative of three experiments. (E) Cells were stained with anti-EEA1, anti-LAMP1, and anti-mTOR. Confocal images representa- tive of two independent experiments are shown. Scale bars, 5 m. (F and G) mTORC1 activities in day 3– and day 5–stimulated naïve CD4+ T cells from young and older in- dividuals after control or VPS39 silencing. The gray histogram represents isotype control. Comparison by two-tailed paired t test (A, B, F, and G). *P < 0.05 and **P < 0.01.
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akt inhibitor iii  (Millipore)
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Fig. 2. SLC7A5-dependent late endosomal mTORC1 activation in naïve CD4+ T cell responses. (A and B) Naïve CD4+ T cells from young and older healthy individuals were activated with anti-CD3/anti-CD28 beads for 3 days in the presence of vehicle or <t>SLC7A5</t> <t>inhibitor</t> JPH203 (top). Alternatively, cells were activated with anti-CD3/ anti-CD28 beads for 5 days with the last 2 days in the presence of vehicle or JPH203 (bottom). mTORC1 activities were determined either by Western blotting of p-S6K1 (A) or by flow cytometry of intracellular p-S6RP (S235/S236) (B). Data are shown as one representative experiment (left) and cumulative data of three or four experiments (right). (C) Naïve CD4+ T cells were activated for 3 days followed by treatment or not with an <t>AKT</t> inhibitor for 2 hours before harvesting. Endosomes were isolated and analyzed for in vitro mTORC1 kinase activity toward S6K1. Total cell lysates (T) and endosome isolates (E) were analyzed by immunoblotting for indicated proteins. Data are shown as one representative of three experiments. (D) In vitro mTORC1 kinase activity of endosome isolates in day 5–stimulated naïve CD4+ T cells from one young and one older individual. Data are shown as one representative of three experiments. (E) Cells were stained with anti-EEA1, anti-LAMP1, and anti-mTOR. Confocal images representa- tive of two independent experiments are shown. Scale bars, 5 m. (F and G) mTORC1 activities in day 3– and day 5–stimulated naïve CD4+ T cells from young and older in- dividuals after control or VPS39 silencing. The gray histogram represents isotype control. Comparison by two-tailed paired t test (A, B, F, and G). *P < 0.05 and **P < 0.01.
Akt Inhibitor Iii, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 2. SLC7A5-dependent late endosomal mTORC1 activation in naïve CD4+ T cell responses. (A and B) Naïve CD4+ T cells from young and older healthy individuals were activated with anti-CD3/anti-CD28 beads for 3 days in the presence of vehicle or SLC7A5 inhibitor JPH203 (top). Alternatively, cells were activated with anti-CD3/ anti-CD28 beads for 5 days with the last 2 days in the presence of vehicle or JPH203 (bottom). mTORC1 activities were determined either by Western blotting of p-S6K1 (A) or by flow cytometry of intracellular p-S6RP (S235/S236) (B). Data are shown as one representative experiment (left) and cumulative data of three or four experiments (right). (C) Naïve CD4+ T cells were activated for 3 days followed by treatment or not with an AKT inhibitor for 2 hours before harvesting. Endosomes were isolated and analyzed for in vitro mTORC1 kinase activity toward S6K1. Total cell lysates (T) and endosome isolates (E) were analyzed by immunoblotting for indicated proteins. Data are shown as one representative of three experiments. (D) In vitro mTORC1 kinase activity of endosome isolates in day 5–stimulated naïve CD4+ T cells from one young and one older individual. Data are shown as one representative of three experiments. (E) Cells were stained with anti-EEA1, anti-LAMP1, and anti-mTOR. Confocal images representa- tive of two independent experiments are shown. Scale bars, 5 m. (F and G) mTORC1 activities in day 3– and day 5–stimulated naïve CD4+ T cells from young and older in- dividuals after control or VPS39 silencing. The gray histogram represents isotype control. Comparison by two-tailed paired t test (A, B, F, and G). *P < 0.05 and **P < 0.01.

Journal: Science immunology

Article Title: Activation of mTORC1 at late endosomes misdirects T cell fate decision in older individuals.

doi: 10.1126/sciimmunol.abg0791

Figure Lengend Snippet: Fig. 2. SLC7A5-dependent late endosomal mTORC1 activation in naïve CD4+ T cell responses. (A and B) Naïve CD4+ T cells from young and older healthy individuals were activated with anti-CD3/anti-CD28 beads for 3 days in the presence of vehicle or SLC7A5 inhibitor JPH203 (top). Alternatively, cells were activated with anti-CD3/ anti-CD28 beads for 5 days with the last 2 days in the presence of vehicle or JPH203 (bottom). mTORC1 activities were determined either by Western blotting of p-S6K1 (A) or by flow cytometry of intracellular p-S6RP (S235/S236) (B). Data are shown as one representative experiment (left) and cumulative data of three or four experiments (right). (C) Naïve CD4+ T cells were activated for 3 days followed by treatment or not with an AKT inhibitor for 2 hours before harvesting. Endosomes were isolated and analyzed for in vitro mTORC1 kinase activity toward S6K1. Total cell lysates (T) and endosome isolates (E) were analyzed by immunoblotting for indicated proteins. Data are shown as one representative of three experiments. (D) In vitro mTORC1 kinase activity of endosome isolates in day 5–stimulated naïve CD4+ T cells from one young and one older individual. Data are shown as one representative of three experiments. (E) Cells were stained with anti-EEA1, anti-LAMP1, and anti-mTOR. Confocal images representa- tive of two independent experiments are shown. Scale bars, 5 m. (F and G) mTORC1 activities in day 3– and day 5–stimulated naïve CD4+ T cells from young and older in- dividuals after control or VPS39 silencing. The gray histogram represents isotype control. Comparison by two-tailed paired t test (A, B, F, and G). *P < 0.05 and **P < 0.01.

Article Snippet: In some experiments, cells were pretreated for 2 hours with 1 M AKT inhibitor (MK-2206 2HCl, Selleckchem) before harvesting.

Techniques: Activation Assay, Western Blot, Flow Cytometry, Isolation, In Vitro, Activity Assay, Staining, Control, Comparison, Two Tailed Test